DNA extraction & magnetic bead technique
DNA extraction is just one of the important and vital experimental steps in the biomedical field. The magnetic grain DNA removal kit utilizes an one-of-a-kind magnetic bead and barrier system. These magnetic grains are particularly covered and have substantial affinity for the target nucleic acid under details problems. When conditions alter, the magnetic grains will certainly launch the adsorbed nucleic acids, achieving the objective of fast splitting up and filtration of high-grade nucleic acids. This efficient magnetic grain modern technology makes it less complicated and extra trusted to remove pure DNA from samples.
(LingJun DNA Extraction kit)
Magnetic bead technique for DNA removal actions:
1) Lysis: First, add the ground and damaged tissue to the lysis option and allow it stand at space temperature level for 5-10 mins.
2) Binding: Then include isopropyl alcohol and magnetic bead suspension to the lysis service and mix by inverting for 5 mins. Then, position the centrifuge tube on a magnetic stand and wait until the magnetic grains entirely adsorb the target DNA before disposing of the supernatant.
3) Rinse: Eliminate the centrifuge tube, include rinse option and vortex to resuspend the magnetic grains. Then, put the centrifuge tube on the magnetic stand once more. Discard the supernatant after the magnetic beads are entirely absorbed. Repeat this step once, guaranteeing that all waste liquid is aspirated, as the residual fluid might influence succeeding experiments. Ultimately, let dry for 2 minutes.
4) Elution: Remove the centrifuge tube, add TE buffer, vortex and pipette the magnetic grains to totally disperse them in the fluid. After that, position it at 56 ° C for 5 minutes, drinking and blending every 2 mins throughout this period to make sure that the magnetic grains are uniformly put on hold. Finally, the DNA is absorbed by the magnetic grains into a new centrifuge tube via a magnetic stand.
( DNA/RNA Extraction)
DNA extraction & comparison of a number of removal techniques
1) Understanding and understanding magnetic bead DNA extraction technology: As the benefits of the magnetic grain approach in genomic DNA removal are progressively becoming a growing number of noticeable, it is suggested to pay close attention to the development of this modern technology and find out how to operate and enhance the magnetic grain DNA removal experimental process.
2) Understand high-throughput and automated devices: As the organic industry’s need for efficiency and automation boosts, familiarity with and proficiency of relevant high-throughput DNA removal instruments and automated systems can boost speculative effectiveness and precision.
3) Safe procedure and experimental effectiveness: Thinking about the danger of exposure to harmful reagents utilizing conventional methods, it is advised to make use of the magnetic bead method for DNA removal to decrease the harm to human wellness during speculative operations and improve speculative efficiency and safety and security.
4) Development and application of research areas: Along with genomic DNA extraction, magnetic bead methods are likewise extensively utilized in illness discovery, genetic medical diagnosis, forensic identification and other fields. It is recommended to explore even more the prospective applications of the magnetic bead method in various biological fields and contribute even more possibilities to the development of the organic market.
( Lingjun Products)
Vendor Introduction
Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction kit.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need gel extraction kit, please feel free to contact us at sales01@lingjunbio.com.
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